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Rilling, Biochem. Biophys. Res. , 1972,46,470. T. R. Green and D. J. , 1972,130,983. K. J. Stone and J. L. Strominger, Proc. Nat. Acad. Sci. , 1972,69, 1287. S. C. Kushwaha, E. L. Pugh, J. K. G. Kramer, and M. Kates, Biochim. Eiophys. Acta, 1972,260, 492; J. K. G. Kramer, S. C. Kushwaha, and M. Kates. Biochim. Eiophys. Actu, 1972, 270, 103. -H. Tai and K. Bloch, J. Biol. , 1972,247, 3767. Biosynthesis 26 However, it is difficult to rationalize, on a chemical basis alone, the stabilization of cation (26) to prosterols such as fusidic acid (27) which have the 2-geometry Direct stabilization of (26) by elimination of the 17P-proton would at give, according to corn forth'^^^ hypothesis, the incorrect geometry about the 17(20)double bond.

Sakamoto, J. Biochem. (Japan), 1969,65, 171. G. F. Gibbons and K. A. Mitropoulos, Biochem. , 1972, 127, 315. 28 Biosynthesis hepatic concentration of cytochrome P-450 does not affect overall hepatic biogenesis of cholester01,~~ with the above results. Whereas the C-4 methyl groups of lanosterol (29) are removed by oxidation to carboxylic acid derivatives and eliminated as carbon dioxide,’. A mechanism (Scheme 1) has been ~uggested’~ for the overall microsomal reaction, which requires NADPH and ’I S.

Smith, Biochem. J . , 1972,130,72P; J. Hubcr. Miiller, and W. Guder, Z . physiul. , 1972, 353, 307. K. W. Gregory, C. Z. Smith, and R. Booth, Biochern. J,, 1972,130, 1163. R. E. Dugan, L. L. Slakey, A. Briedis. and J. W. Porter. Arch. Biochem. ,1972, 152,21. A. Edwards and R. G. Gould, 3. Biol. , 1972,247,1520. M. Higgins, T. Kawachi, and H. Rudney, Biochem. Biophys. Res. 45138. Biosynthesis of niterpenes, Steroids, and Carotenoids 19 indicating that the circadian rhythm is due solely to changes in the rate of synthesis of the enzyme and not to the rate of degradation.

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