By Ehud Keinan
Exploiting the inherent combinatorial mechanism within the biosynthesis of antibodies, a nearly unlimited number of biocatalysts should be generated. Catalytic antibodies are able to acting virtually any kind of response with excessive selectivity and stereospecificity.Here, the pioneers within the use of catalytic antibodies evaluate the complete scope of this interdisciplinary box, protecting such issues as:theoretical elements of constitution, mechanism and kineticspractical concerns, from immunization options to screening methodsin vitro evolution and different smooth approachesapplications from natural synthesis to clinical uses.Backed by means of the best experts in antibody catalysis, this can be the 1st booklet to supply such complete insurance and may be the best reference for biochemists, natural chemists, biotechnologists and biomedical researchers.
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Extra resources for Catalytic Antibodies
J. Biol. Chem. 274 (1999), p. 15967–70 Arevalo, J. , Taussig, M. , Wilson, I. , Nature 365 (1993), p. , Biochemistry 31 (1992), p. 6884–97 Haynes, M. , Israel J. Chem. 136 (1996), p. 151 Kim, H. , J. Immunol. 143 (1989), p. 1 Introduction Antibody molecules elicited with rationally designed transition-state analogs catalyze numerous reactions, including many that cannot be achieved by standard chemical methods. Although relatively primitive when compared with natural enzymes, these catalysts are valuable tools for probing the origins and evolution of biological catalysis.
Not only do studies of catalytic antibodies provide insights into the evolution of the binding and catalytic function of enzymes, they also provide fundamental insights into the molecular basis of the immune response itself. Over half a century ago there was considerable debate over the mechanisms by which the immune system is able to evolve selective, high affinity receptors for an almost infinite number of ligands. Once it was established that the immune system produces a large number of antibodies with different sequences through recombination and somatic mutation, sequence diversity was widely accepted as the basis for the tremendous binding potential of antibody repertoire.
Residues making important packing interactions with jeffamine in the antibody combining site are also shown. This forces a conformational change of CDRH1 to avoid steric clashes between the phenol residue of Tyr27H and the carboxyl group of Asp96H . As a result of antibody combining site reorganization, two different sets of residues are used by the germline antibody to interact with either NMP or jeffamine (Fig. 10). The charge distribution and the shape of the antibody combining site in the germline Fab-jeffamine complex is very different from the that of the same antibody when there is no ligand bound or with its hapten NMP bound (Fig.
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