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Additional resources for Cellular Mechanisms of Renal Tubular Ion Transport
33 33 35 37 38 38 40 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 1 . . . . . . . . . . . 41 45 . 46 INTRODUCTION An ion-selective electrode records a potential which is closely related to the chemical potential of that ion to which the electrode is sensitive. In cells and tissues where there are gradients in both the electrical and chemical potential, one must know the electrical potential at the tip of the electrode to obtain the chemical potential alone.
23 24 25 28 28 INTRODUCTION The intracellular pH (pH,) of snail neurons appears to be regulated by a mechanism which involves the simultaneous influx of Na+ and HC0,ions, and the efflux of CI- and Hf ions (7, 8). There may be a single carrier in the cell membrane with separate sites for each of these ions, as illustrated in Fig. 1. The main evidence for this scheme can be summarized as follows: (a) pH, recovery after internal acidification is accompanied by an increase in internal Na’ and inhibited by removal of external Na+; (b) pHi recovery is accompanied by a decrease in internal CI- and inhibited by depletion of internal Cl-; (c) pHi recovery is inhibited by the removal of HC03- and by the anion-exchange inhibitor 4-acetamido-4’isothiocyanatostilbene-2,2’-disulfonicacid (SITS) (6-8).
Sci. Exutes N u t . 6, 118-126. 18. , and Thomas, R. C. (1976). Microelectrode measurement of the intracellular pH of mammalian heart cells. Nature (London) 262, 224-225. 19. , and Thomas, R. C. (1976). Direct measurement of the intracellular pH of mammalian cardiac muscle. J. Physiol. (London) 262, 755-771. 20. Fenn, W. 0.. and Maurer, F. W. (1935). The pH of muscle. Protoplasma 24, 337-345. 21. Fridericia, L. S. (1920). Exchange of chloride ions and of carbon dioxide between blood corpuscles and blood plasma.
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