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By J. Cervós-Navarro, J. Artigas, B. J. Mršulja (auth.), Prof. Dr. Konstantin-Alexander Hossmann, Dr. Igor Klatzo (eds.)

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Additional resources for Cerebrovascular Transport Mechanisms: International Congress of Neuropathology, Vienna, September 5–10, 1982

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Histoehemie 6: 254-259 6. Mayahara H, Hirano H, Saito T, Ogawa K (1967) The new lead eitrate method for the ultraeytoehemieal demonstration of aetivity of non-speeifie alkaline phosphatase (orthophosphorie monoesterphoshydrolase). Histoehemie 11: 88-96 7. Waehstein M, Meisel E (1957) Histoehemistry of hepatie phosphatases at a physiologie pH with speeial referenee to the demonstration of bile eanalieuli. Amer J elin Path 27: 13-23 39 8. Kreutzberg GW, Barron KD, Schubert P (1978) Cytochemical localization of 5'nucleotidase in glial plasma membranes.

This enzyme disappears from the neuropil whieh represents mainly binding sites at the postsynaptie membranes. However, within 2 weeks all eapillaries of the faeial nueleus area beeome positive. When viewed with the eleetron mieroseope, enzyme aetivity is present in the basal laminae and the interendothelial spaees of the ab luminal portion up to the tight junetions. Referenees 1. Dempsey EW, Wisloeki GB (1955) An eleetron mieroseopie study of the blood-brain barrier of the rat, employing silver nitrate as a vital stain.

When the barrier is opened, the number of pits, vesicles and tubules increases. Such cerebral endothelium resembles normal endothelium of certain fish where numerous membrane invaginations do not signify vesicular or tubular transport. However, such transport has not been entirely ruled out in reactive endothelium. Another route of exudation during barrier opening may be via patent endothelial junctions, especially during intravascular infusion of hyperosmotic solutions. The permeability of the tight junctions, however, is not reflected unequivocally by its intramembranous structure.

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